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Dr. Lukyanetz E.A. group: MECHANISMS OF EXOCYTOSIS
The studies of Dr. E. A. Lukyanetz’s group are concentrated on such processes as intracellular calcium signaling and calcium-dependent exocytosis in neuronal and endocrine cells. Especial attention is paid to the role of synaptic proteins in the processes of synaptic transmission. Variety of such proteins has been already described in distinct preparations; however, their precise localization and role in vesicular trafficking during functional changes of the cells remain ambiguous. Besides that, new synaptic proteins appear to be involved in the chain of the vesicular cycle, a function of which remains unclear. Therefore, our group studies the role of synaptic proteins in trafficking of secretory vesicles either core SNARE proteins - vesicular (synaptobrevin/VAMPs) and plasma membrane (syntaxins/SNAP-25) synaptic proteins or novel presynaptic proteins (Munc-13, Munc-18, CAPS, Csp and Doc2) during cell excitation. We use the methods which allow to measure simultaneously the following parameters: calcium current, membrane capacity, changes in intracellular calcium concentration, amperometric measurements of secretory events and molecular biology approaches. |
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