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| − | ='''Our research'''=
| + | [https://new.biph.kiev.ua/en/home/structure/departments/department-of-cellular-membranology/ Read more...] |
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| − | Our Department research is focused on the role of membrane proteins, mainly ion channels and metabotropic receptors, in the functioning of single neurons, neural networks, and the whole organs. The current research topics of the Department are:
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| − | * biophysical and pharmacological properties of ion channels and receptors of cellular membranes (plasmalemma and nuclear envelope);
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| − | * molecular mechanisms of epilepsy and epileptogenesis and pharmacological agents with antiseizure properties;
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| − | * role of membrane receptors and ion channels in synaptic plasticity, learning, integral activity of nervous system and behavior;
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| − | * peripheral and central mechanisms of pain and possible ways of its correction;
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| − | * using of stem cells for the treatment of neurological disorders (Alzheimer’s, Parkinson’s disease, epilepsy, and ischemic stroke) and
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| − | traumatic injuries of the spinal cord.
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| − | ='''Methods'''=
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| − | Highly qualified Departments’ staff has strong expertise in virtually all electrophysiological techniques, various disease models and cell/tissue cultural techniques allowing studying physiological functions and some pathologies from molecular to system levels including:
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| − | * pharmacological patch-clamp studies of different ion channels in acutely isolated cells (pyramidal neurons from hippocampus, cortex, and hypothalamus; Purkinje and granule neurons from cerebellum; spinal dorsal horn neurons; primary sensory neurons; glial cells), cultured cells (the same as above; cancer cell lines) and heterologous expression systems (HEK293, CHO, and PC-12 lines);
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| − | * registration of electrical activity of single neurons, local field potentials, EEG in freely behaving animals;
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| − | * isolation and cultivation of stem cells and tissue engineering;
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| − | * electrophysiological studies of primary nociception ex vivo and in vivo;
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| − | * seizure-like activity in acute isolated and cultured brain slices in various models of epilepsy as well as intracranial EEG in various in vivo models of epilepsy;
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| − | * a wide range of behavioral tests (open field, different mazes, Morris water maze, etc) that allow us to study general locomotor activity, anxiety and depression-like behavior, learning and memory, social behavior, etc.
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| − | [[File:Depcm2020.png|border|800px]]
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| − | Collage (clockwise): ''The effect of ASIC1a inhibition on hippocampal LTP (Buta A et. al, J. Med. Chem. 2015); patch-clamp technique (V. Kulyk); confocal microscopy of neural stem cell culture and neurospheres - astrocytes are red, neurons blue (O. Rybachuk); brain structures' LFPs in freely moving rat and it's spectrogram (R. Bohovyk); meeting colleagues at FENS Forum (A. Cherninskyi and E. Knapska); making artificial cerebrospinal fluid (O. Iegorova); central — specimens' microscopy (M. Fedoriuk).''
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| − | ='''Members'''=
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| − | '''Research staff'''
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| − | Prof. Oleg Krishtal - the head of department, PhD, DSc, Professor, Member of Academia Europaea, Full Member of the National Academy of Sciences of Ukraine
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| − | Dr. Oleksandr Maximyuk - leading researcher, PhD
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| − | Dr. Maksim Storozhuk - leading researcher, PhD, Dsc
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| − | Dr. Dmytro Isaev - leading researcher, PhD
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| − | Dr. Ihor Chizhmakov - senior researcher, PhD
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| − | Dr. Andrii Cherninskyi - senior researcher, PhD
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| − | Dr. Anna Kotliarova - senior researcher, PhD
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| − | Dr. Alina Savotchenko - researcher, PhD
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| − | Dr. Olena Iegorova - researcher, MD, PhD
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| − | Dr. Viacheslav Kulyk - researcher, PhD
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| − | Dr. Oksana Rybachuk - researcher, PhD
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| − | Dr. Dariia Dryn - researcher, PhD
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| − | Dr. Mariia Melnyk - researcher, PhD
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| − | Tetiana Volkova - researcher
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| − | Andrii Buta - researcher
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| − | Liudmila Nikolaienko - junior researcher
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| − | Olha Zapukhliak - junior researcher
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| − | Volodymyr Khmyz - junior researcher
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| − | Yurii Tkachenko - junior researcher
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| − | Artur Romanov - junior researcher
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| − | Oleksii Lunko - junior researcher
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| − | Olesia Lunko - junior researcher
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| − | Ruslan Bohovyk - junior researcher
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| − | Mykhailo Fedoriuk - junior researcher
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| − | Olena Kotyk - junior researcher
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| − | '''Technical stuff and secretary'''
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| − | Artem Venhreniuk
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| − | '''PhD students'''
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| − | Sofiia Demchenko
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| − | Mariia Stefanenko
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| − | Marharyta Semenikhina
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| − | Oksana Nikolaienko
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| − | Pavlo Novikov
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| − | Iuliia Nesterenko
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| − | ='''Publications'''=
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| − | Li, HS; Su, XY; Song, XL; Qi, X; Li, Y; Wang, RQ; Maximyuk, O; Krishtal, O; Wang, TT; Fang, HQ; Liao, LJ; Cao, H; Zhang, YQ; Zhu, MX; Liu, MG; Xu, TL [https://doi.org/10.1523/JNEUROSCI.0213-19.2019 Protein Kinase C Lambda Mediates Acid-Sensing Ion Channel 1a-Dependent Cortical Synaptic Plasticity and Pain Hypersensitivity] // JOURNAL OF NEUROSCIENCE, 39 (29), 2019, P. 5773-5793 [https://doi.org/10.1523/JNEUROSCI.0213-19.2019 DOI: 10.1523/JNEUROSCI.0213-19.2019]
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| − | Rybachuk, O; Kopach, O; Pivneva, T; Kyryk, V [https://doi.org/10.21769/BioProtoc.3165 Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue] // BIO-PROTOCOL, 9 (4), 2019, Article Number: e3165 [https://doi.org/10.21769/BioProtoc.3165 DOI: 10.21769/BioProtoc.3165]
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| − | Semenikhina, M; Bogovyk, R; Fedoriuk, M; Nikolaienko, O; Al Kury, LT; Savotchenko, M; Krishtal, O; Isaeva, E [https://doi.org/10.1016/j.neulet.2018.10.058 Inhibition of protease-activated receptor 1 ameliorates behavioral deficits and restores hippocampal synaptic plasticity in a rat model of status epilepticus] // NEUROSCIENCE LETTERS, 692, 2019, P. 64-68 [https://doi.org/10.1016/j.neulet.2018.10.058 DOI: 10.1016/j.neulet.2018.10.058]
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| − | Kopach, O; Rybachuk, O; Krotov, V; Kyryk, V; Voitenko, N; Pivneva, T [https://doi.org/10.1242/jcs.210989 Maturation of neural stem cells and integration into hippocampal circuits - a functional study in an in situ model of cerebral ischemia] // JOURNAL OF CELL SCIENCE, 131 (4), 2018, pii: jcs210989 [https://doi.org/10.1242/jcs.210989 DOI: 10.1242/jcs.210989]
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| − | Luo, JL; Qian, AH; Oetjen, LK; Yu, WH; Yang, P; Feng, J; Xie, ZL; Liu, SB; Yin, SJ; Dryn, D; Cheng, JZ; Riehl, TE; Zholos, AV; Stenson, WF; Kim, BS; Hu, HZ [https://doi.org/10.1016/j.immuni.2018.04.021 TRPV4 Channel Signaling in Macrophages Promotes Gastrointestinal Motility via Direct Effects on Smooth Muscle Cells] // IMMUNITY, 49 (1), 2018, P. 107-119 [https://doi.org/10.1016/j.immuni.2018.04.021 DOI: 10.1016/j.immuni.2018.04.021]
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| − | Kononenko, O; Galatenko, V; Andersson, M; Bazov, I; Watanabe, H; Zhou, XW; Iatsyshyna, A; Mityakina, I; Yakovleva, T; Sarkisyan, D; Ponomarev, I; Krishtal, O; Marklund, N; Tonevitsky, A; Adkins, DL; Bakalkin, G [https://doi.org/10.1096/fj.201601039R Intra- and interregional coregulation of opioid genes: broken symmetry in spinal circuits] // FASEB JOURNAL, 31 (5), 2017, P. 1953-1963
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| − | [https://doi.org/10.1096/fj.201601039R DOI: 10.1096/fj.201601039R]
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| − | Kononenko, O; Bazov, I; Watanabe, H; Gerashchenko, G; Dyachok, O; Verbeek, DS; Alkass, K; Druid, H; Andersson, M; Mulder, J; Svenningsen, AF; Rajkowska, G; Stockmeier, CA; Krishtal, O; Yakovleva, T; Bakalkin, G [https://doi.org/10.1016/j.bbagen.2016.11.002 Opioid precursor protein isoform is targeted to the cell nuclei in the human brain] // BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1861 (2), 2017, P. 246-255 [https://doi.org/10.1016/j.bbagen.2016.11.002 DOI: 10.1016/j.bbagen.2016.11.002]
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| − | Feng, J; Yang, P; Mack, MR; Dryn, D; Luo, JL; Gong, X; Liu, SB; Oetjen, LK; Zholos, AV; Mei, ZN; Yin, SJ; Kim, BS; Hu, HZ [https://doi.org/10.1038/s41467-017-01056-8 Sensory TRP channels contribute differentially to skin inflammation and persistent itch] // NATURE COMMUNICATIONS, 8, 2017, Article Number: 980 [https://doi.org/10.1038/s41467-017-01056-8 DOI: 10.1038/s41467-017-01056-8]
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| − | Storozhuk, M; Kondratskaya, E; Nikolaenko, L; Krishtal, O [https://doi.org/10.1186/s13041-016-0269-4 A modulatory role of ASICs on GABAergic synapses in rat hippocampal cell cultures] // MOLECULAR BRAIN, 9, 2016, Article Number: 90 [https://doi.org/10.1186/s13041-016-0269-4 DOI: 10.1186/s13041-016-0269-4]
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| − | Liu, MG; Li, HS; Li, WG; Wu, YJ; Deng, SN; Huang, C; Maximyuk, O; Sukach, V; Krishtal, O; Zhu, MX; Xu, TL [https://doi.org/10.1038/srep23350 Acid-sensing ion channel 1a contributes to hippocampal LTP inducibility through multiple mechanisms] // SCIENTIFIC REPORTS, 6, 2016, Article Number: 23350 [https://doi.org/10.1038/srep23350 DOI: 10.1038/srep23350]
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| − | Tesoriero, C; Codita, A; Zhang, MD; Cherninsky, A; Karlsson, H; Grassi-Zucconi, G; Bertini, G; Harkany, T; Ljungberg, K; Liljestrom, P; Hokfelt, TGM; Bentivoglio, M; Kristensson, K [https://doi.org/10.1073/pnas.1521463112 H1N1 influenza virus induces narcolepsy-like sleep disruption and targets sleep-wake regulatory neurons in mice] // Proceedings Of The National Academy Of Sciences Of The United States Of America, 113 (3), 2016, E368-E377 [https://doi.org/10.1073/pnas.1521463112 DOI: 10.1073/pnas.1521463112]
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| − | McGarvey L.P., Butler C.A., Stokesberry S., Polley L., McQuaid S., Abdullah H., Ashraf S., McGahon M.K., Curtis T.M., Arron J., Choy D., Warke T.J., Zholos, A Bradding P., Ennis M., Costello R., Heaney L.G. [https://doi.org/10.1016/j.jaci.2013.09.016 Increased expression of bronchial epithelial transient receptor 1 potential vanilloid 1 channels in severe asthma] // Journal of Allergy and Clinical Immunology 133 (3), 2014, 704-712
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| − | [https://doi.org/10.1016/j.jaci.2013.09.016 DOI: 10.1016/j.jaci.2013.09.016]
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| − | Krishtal O [https://doi.org/10.1016/j.neuropharm.2014.12.014 Receptor for protons: First observations on Acid Sensing Ion Channels] // NEUROPHARMACOLOGY, 94, 2015, P. 4-8 [https://doi.org/10.1016/j.neuropharm.2014.12.014 DOI: 10.1016/j.neuropharm.2014.12.014]
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| − | Ievglevskyi O, Isaev D, Netsyk O, Romanov A, Fedoriuk M, Maximyuk O, Isaeva E, Akaike N, Krishtal O [https://doi.org/10.1098/rstb.2015.0431 Acid-sensing ion channels regulate spontaneous inhibitory activity in the hippocampus: possible implications for epilepsy] // The Royal Society Phil. Trans. R. Soc. B, 2016, 371(1700). pii: 20150431 [https://doi.org/10.1098/rstb.2015.0431 DOI: 10.1098/rstb.2015.0431]
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| − | Buta A, Maximyuk O, Kovalskyy D, Sukach V, Vovk M, Isaev D, Ievglevskyi O, Isaeva E, Savotchenko A, Krishtal O [https://doi.org/10.1021/jm5017329 Novel potent orthosteric antagonist of ASIC1a prevents NMDAR-dependent LTP induction] // J Med Chem. 2015 Jun 11;58(11):4449-61 [https://doi.org/10.1021/jm5017329 DOI: 10.1021/jm5017329]
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