Department of Cytology

Інститут фізіології ім. О. О. Богомольця
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Department of Cytology has been established in 1996 based on the Laboratory of Neurocytology. Its organizer and the only chair until nowadays is [G.G.Skibo|Prof. Galyna Skibo], the corresponding member of NAS of Ukraine, laureate of State Prize of Ukraine in sciences and engineering, Bogomoletz and Kostiuk Prizes of NAS of Ukraine.


Our research

The research activity of the Department of Cytology is focused on the study of cellular and molecular mechanisms of neurodegeneration in different parts of the nervous system and the search for neuroprotective approaches. The current research topics of the Department are:

  • genetically determined molecular mechanisms of intercellular and intracellular signaling in norm and pathology in vitro (brain ischemia, Alzheimer’s disease)
  • molecular and cellular mechanisms of endogenous neuroprotection in the models of neurodegenerative diseases in vivo and in vitro (brain ischemia)
  • regenerative properties of different origin stem cells and peculiarities of their application at experimentally induced neurodegenerative pathologies in vivo (brain ischemia, perinatal CNS pathology, neuroinflammation)
  • development of biosafety criteria for stem cell cultivation
  • cell mechanisms driving brain tissue degeneration in the rotenone model of Parkinson's disease
  • dependence of structural and functional features of the brain on the function of the gastrointestinal tract (metabolic syndrome, exocrine pancreatic insufficiency) in vivo and in vitro
  • effect of substances with antioxidant properties on the nervous tissue recovery of experimental animals during the brain ischemia-reperfusion

  • Synapses with continuous (A) and perforated (B) postsynaptic densities (red lines) between host dendritic spines (ds, brown) and grafted GFP+/DAB+ presynaptic axon terminals (at, green) in the contralateral somatosensory cortex of stroke-injured rats. (Scale bar – 0.2 μm) Source
  • The GIF animation created using serial electron micrographs of the hippocampal CA1 area, illustrating the direct interaction of the pyramidal neuron and astrocyte by exocytosis/endocytosis (to see that animation, open full image) Source
  • FGF-2-Overexpressing Neural Stem/Progenitor Cells (FGF-2-NPCs) made contact with blood vessels at sites with astrocyte coverage, but no pericytes Source
  • Synapses in СА1 stratum radiatum in vivo Red arrow-simple, yellow-multiple, green-perforated Source
  • 3D reconstructions of spine synapses contacted by glia under ischemic conditions Source

Research objects

  • Animals: rats, mice, gerbils, pigs;
  • Cell cultures: organotypic cultures of the hippocampus, dissociated cultures of CNS neurons, enteric neuron cultures.

Research methods

  • modelling of neurodegenerative states in vivo (cerebral ischemia, Parkinson’s disease, neuroinflammation, perinatal pathology of CNS, metabolic syndrome, exocrine pancreatic insufficiency) and in vitro (cerebral ischemia, Alzheimer’s disease, stress);
  • light, confocal, and transmission electron microscopy;
  • immunohistochemistry;
  • behavioral tests (open field, balance beam walking, Morris water maze, T- maze)
  • immunoblotting;
  • morphometry: computerized image analysis, quantitative ultrastructural analysis.


  • Lund Stem Cell Center, Lund University, Lund, Sweden;
  • Food for Health Science Center, Lund University, Lund, Sweden;
  • Chebotarev State Institute of Gerontology NAMS of Ukraine, Kyiv, Ukraine;
  • Oles Honchar Dnipro National University, Dnipro, Ukraine.


  1. Galyna Skibo – Prof., DSc, corresponding member of NAS of Ukraine, head of Department,
  2. Oleksandr Nikonenko – DSc, leading researcher,
  3. Oleh Tsupykov – DSc, leading researcher,
  4. Iryna Lushnikova – DSc, leading researcher,
  5. Tetiana Kovalenko – Candidate of sciences (PhD), senior researcher,
  6. Iryna Osadchenko – Candidate of sciences (PhD), senior researcher,
  7. Olena Mankivska – Candidate of sciences (PhD), senior researcher
  8. Kateryna Yatsenko – DSc, senior researcher,
  9. Maryna Patseva – Candidate of sciences (PhD), researcher,
  10. Olena Savchuk – Candidate of sciences (PhD), junior researcher,
  11. Kateryna Smozhanyk – technician.
  12. Petro Maiorenko – technician.
  13. Liudmyla Melnychuk - laboratory assistant.

PhD students

  1. Nataliia Chaika -
  2. Olha Kostiuchenko -
  3. Nadiia Kravchenko -
  4. Dmytro Shepilov -

Recent publications

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