Department of Cytology

Інститут фізіології ім. О. О. Богомольця
(Difference between revisions)
Jump to: navigation, search
(smth to begin with)
 
(17 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
[[File:skibo.jpg|200px|thumb|G.G. Skibo|left]] Department of Cytology was organized in 1996 on the basis of laboratory neurocytology. Constant head of department and head of the research is MD, professor, winner of O.O. Bogomolets premium, Honored Worker of Science of Ukraine Galina G. Skibo.
+
Department of Cytology has been established in 1996 based on the Laboratory of Neurocytology. Its organizer and the only chair until nowadays is [G.G.Skibo|Prof. Galyna Skibo], the corresponding member of NAS of Ukraine, laureate of State Prize of Ukraine in sciences and engineering, Bogomoletz and Kostiuk Prizes of NAS of Ukraine.
  
−
== Science ==
+
== Our research  ==
−
Science Department of Cytology aims to study the molecular and cellular mechanisms of nervous tissue damage arising from the development of neurodegenerative diseases (cerebral ischemia, Alzheimer's disease, Parkinson's disease) or accompanying pathological states (perinatal pathology of the central nervous system, brain injury, stress, exocrine pancreatic failure), and study approaches to the prevention and correction of neurodegenerative processes. An important part of research is testing pharmacological agents to identify their neuroprotective properties. Started studying the possibilities of using embryonic nervous tissue and stem cells to correct neurodegenerative processes caused by cerebral ischemia.
+
  
−
[[File:Synapsys.jpg|300px|thumb|Types of synapses red arrow - simple, yellow - perforated, green - multiple]] Scientists department found that short-term cerebral ischemia in model systems (in vitro and in vivo) causes the development of destructive changes of neurons and synaptic reorganization of staff in CA1 area of the hippocampus - a brain structure that is responsible for the processes of memory formation and learning and is highly sensitive to lack of oxygen and glucose. When using light and electron microscopy were investigated structural characteristics of delayed neuronal death of hippocampal CA1 area in ischemic effects of varying duration and period of reperfusion. Established in early periods of ischemic damage occurs synaptic remodeling apparatus, namely changing the spatial distribution of synaptic vesicles at presynaptic terminals, increasing the thickness and density of postsynaptic podovzhenist, changing the ratio of types of synaptic terminals upwards and multiple perforated significantly increased volume astrohlialnyh processes and their contact area of pre- and postsynaptic elements synapses. These data suggest that ischemic effect induces synaptic plastic adjustment device, and a clear correlation between changes in structural elements confirms the concept of "tripartite synapse", according to which astrohliyiyi processes that envelop the synapse, the third functional component with presynaptic and postsynaptic bud terminallyu.
+
The research activity of the Department of Cytology is focused on the study of cellular and molecular mechanisms of neurodegeneration in different parts of the nervous system and the search for neuroprotective approaches. The current research topics of the Department are:
 +
* genetically determined molecular mechanisms of intercellular and intracellular signaling in norm and pathology in vitro (brain ischemia, Alzheimer’s disease)
 +
* molecular and cellular mechanisms of endogenous neuroprotection in the models of neurodegenerative diseases in vivo and in vitro (brain ischemia)
 +
* regenerative properties of different origin stem cells and peculiarities of their application at experimentally induced neurodegenerative pathologies in vivo (brain ischemia, perinatal CNS pathology, neuroinflammation)
 +
* development of biosafety criteria for stem cell cultivation
 +
* cell mechanisms driving brain tissue degeneration in the rotenone model of Parkinson's disease
 +
* dependence of structural and functional features of the brain on the function of the gastrointestinal tract (metabolic syndrome, exocrine pancreatic insufficiency) in vivo and in vitro
 +
* effect of substances with antioxidant properties on the nervous tissue recovery of experimental animals during the brain ischemia-reperfusion
 +
<br clear=all>
  
−
It was discovered structural and functional connection between the manifestations of destructive changes of neurons and glial reactivity level cells (microglia hyperplasia and hypertrophy of astrocytes) in ischemic hippocampal impact.
+
----
−
[[File:Hippocamp.jpg|250px|thumb|Three-dimensional reconstruction to study synaptic plasticity of hippocampal neurons and glial response to ischemic lesions|left]] In the model of cultured hippocampal slices was shown that a shortage of oxygen and glucose pyramidal neurons of the hippocampus CA1 area are the most vulnerable. Intercalary, astrohlialni and microglial cells compared were more resistant to oxygen-glucose derivation (SRM), moreover, their activation is observed, indicating the potential for interneurons and glia modulate pyramidal neurons function and affect the functioning of the system. Shows the change in the level of expression of subunits HIF-1α, HIF-3α, SERCA2b and PMCA1-2 in neurons and CA1 hippocampal area SA3 in response to holding SRM and anoksychnoho prekondytsionuvannya and reduce the number of damaged cells in both areas of the hippocampus with a stabilization of HIF-1.
+
 
−
It developed an original computer program image analysis using simulation in which cerebral ischemia in vivo and in vitro was investigated synaptic changes in the spatial distribution of vesicles in the terminal area CA1 of the hippocampus. It is shown that after an ischemic episode spatial cluster formed vesicles become more diffuse, and most organelles were removed from the active zone of the synapse.
+
<div><ul>
−
In the study of correction of ischemic brain damage data were obtained regarding the neuroprotective action of aromatic amino acids, FGL - peptide mimetics NCAM, bioflavonoids, including Corvitin. In terms of experimental cerebral ischemia was found neuroprotective effect of hypoxic pre- and postkondytsionuvannya the state CA1 area of the hippocampus.
+
<li style="display: inline-block;"> [[File:Dept_cytol_01.jpeg|thumb|none|300px|Synapses with continuous (A) and perforated (B) postsynaptic densities (red lines) between host dendritic spines (ds, brown) and grafted GFP+/DAB+ presynaptic axon terminals (at, green) in the contralateral somatosensory cortex of stroke-injured rats. (Scale bar – 0.2 μm) [https://doi.org/10.1073/pnas.2000690117 Source] ]] </li>
−
It was found dependent morphological and functional state of neurons of the hippocampus of exocrine pancreatic function and the data on the possibility of correcting pancreatic encephalopathy using enzyme replacement therapy.
+
<li style="display: inline-block;"> [[File:Dept_cytol_02.gif |thumb|none|300px|The GIF animation created using serial electron micrographs of the hippocampal CA1 area, illustrating the direct interaction of the pyramidal neuron and astrocyte by exocytosis/endocytosis (to see that animation, open full image) [https://doi.org/10.1007/s12264-021-00725-5 Source] ]] </li>
−
[[File:Stemcell.jpg|300px|thumb|The integration of stem cells in the nervous tissue in ischemic damage]] In model systems in vivo and in vitro study was conducted regenerative potential of stem cells of different origins in ischemic lesions. It is shown that stem cells transplanted after experimental ischemic impact in both models are able to survive in the long term the recipient tissue, differentiate into mature neurons as well as in cells astrohliyi, and integrate into the fabric of the recipient. Established that stereotactic transplantation neural progenitor cells promotes restoration of spatial memory in animals after ischemic brain damage.
+
<li style="display: inline-block;"> [[File:Dept_cytol_03.jpeg|thumb|none|300px|FGF-2-Overexpressing Neural Stem/Progenitor Cells (FGF-2-NPCs) made contact with blood vessels at sites with astrocyte coverage, but no pericytes  [https://doi.org/10.3727%2F096368916X690421 Source] ]] </li>
−
When the clinic in patients with ischemic stroke frequency of allelic polymorphism studied genes encoding subunits of constitutional and imunoproteasom. When played ischemic stroke in vivo measurements were performed three types of proteolytic activity of the proteasome (trypsynopodibna, himotrypsynopodibna and peptydhlutamil peptydhidrolazna) in the brain tissue of rats. These data suggest proteasome dysfunction in conditions of ischemic stroke.
+
<li style="display: inline-block;"> [[File:Dept_cytol_04.jpeg|thumb|none|300px|Synapses in СА1 stratum radiatum in vivo
 +
Red arrow-simple, yellow-multiple, green-perforated [https://doi.org/10.1002/hipo.20211 Source] ]] </li>
 +
<li style="display: inline-block;"> [[File:Dept_cytol_05.jpeg|thumb|none|300px|3D reconstructions of spine synapses contacted by glia under ischemic conditions [https://doi.org/10.1002/hipo.20551 Source] ]] </li>
 +
</ul></div>
 +
 
 +
==Research objects==
 +
* Animals:  rats, mice, gerbils, pigs;
 +
* Cell cultures: organotypic cultures of the hippocampus, dissociated cultures of CNS neurons, enteric neuron cultures.
 +
 
 +
==Research methods==
 +
* modelling of neurodegenerative states in vivo (cerebral ischemia, Parkinson’s disease, neuroinflammation, perinatal pathology of CNS, metabolic syndrome, exocrine pancreatic insufficiency) and in vitro (cerebral ischemia, Alzheimer’s disease, stress);
 +
* light, confocal, and transmission electron microscopy;
 +
* immunohistochemistry;
 +
* behavioral tests (open field, balance beam walking, Morris water maze, T- maze)
 +
* immunoblotting;
 +
* morphometry: computerized image analysis, quantitative ultrastructural analysis.
 +
 
 +
==Collaboration==
 +
* Lund Stem Cell Center, Lund University, Lund, Sweden;
 +
* Food for Health Science Center, Lund University, Lund, Sweden;
 +
* Chebotarev State Institute of Gerontology NAMS of Ukraine, Kyiv, Ukraine;
 +
* Oles Honchar Dnipro National University, Dnipro, Ukraine.
  
 
== Team ==
 
== Team ==
−
=== Stuff ===
 
−
# Skibo G.G. - Head of Department, MD, Prof.
 
−
# Kovalenko T.M. – PhD, Leading Researcher
 
−
# Pivneva T.A. – PhD, Leading Researcher 
 
−
# Nikonenko A.G. – PhD, Leading Researcher
 
−
# Tsupykov O.M – PhD, Leading Researcher
 
−
# Voitenko L.P. – PhD, Leading Researcher
 
−
# Lushnikova I.V. – PhD, Senior Researcher
 
−
# Osadchenko I.O. – PhD, Senior Researcher 
 
−
# Patseva М.А. – PhD, Researcher 
 
−
# Maistrenko A.M. – Junior Researcher
 
−
# Nikandrova E.O. – Junior Researcher
 
−
# Rybachuk O.A. – Junior Researcher
 
−
# Savchuk O.I. – Junior Researcher
 
−
# Smozhanyk K.G. – Lead Engineer
 
−
# Mayorenko P.P. – Lead Engineer 
 
−
# Melnychuk L.P. - Senior Laboratory
 
  
−
=== Graduate students ===
+
# Galyna Skibo – Prof., DSc, corresponding member of NAS of Ukraine, head of Department,    skibo@biph.kiev.ua
−
# Goncharova K.
+
# Oleksandr Nikonenko – DSc, leading researcher,  agn@biph.kiev.ua
−
# Zabenko E.
+
# Oleh Tsupykov – DSc, leading researcher,  tsupykov@gmail.com
−
# Maleyeva G.
+
# Iryna Lushnikova – DSc, leading researcher,  li@biph.kiev.ua
 +
# Tetiana Kovalenko – Candidate of sciences (PhD), senior researcher,  tnk@biph.kiev.ua
 +
# Iryna Osadchenko – Candidate of sciences (PhD), senior researcher,  osad@biph.kiev.ua
 +
# Olena Mankivska – Candidate of sciences (PhD), senior researcher  emankovskaya@biph.kiev.ua
 +
# Kateryna Yatsenko – DSc, senior researcher,  kateryna.yatsenko@gmail.com
 +
# Maryna Patseva – Candidate of sciences (PhD), researcher,  pma@biph.kiev.ua
 +
# Olena Savchuk – Candidate of sciences (PhD), junior researcher,  floweringbowl@ukr.net
 +
# Kateryna Smozhanyk – technician.
 +
# Petro Maiorenko – technician.
 +
# Liudmyla Melnychuk - laboratory assistant.
 +
 
 +
===PhD students===
 +
 
 +
# Nataliia Chaika -  nataliia_chaika@ukr.net
 +
# Olha Kostiuchenko -  kostiuchenko.olha@biph.kiev.ua
 +
# Nadiia Kravchenko -  kravchenko.nadiia@biph.kiev.ua
 +
# Dmytro Shepilov -  shepilov@biph.kiev.ua
 +
 
 +
== Recent publications ==
 +
 
 +
* Shepilov D, Kovalenko T, Osadchenko I, Smozhanyk K, Marungruang N, Ushakova G, Muraviova D, Hållenius F, Prykhodko O, Skibo G. [https://doi.org/10.3389/fnut.2022.565051 Varying Dietary Component Ratios and Lingonberry Supplementation May Affect the Hippocampal Structure of ApoE-/- Mice.] Front Nutr. 2022 Feb 16;9:565051. doi.org/10.3389/fnut.2022.565051
 +
 
 +
* Mankivska, O.P., Chaika, N.V. & Skibo, G.G. [https://doi.org/10.1007/s11062-022-09918-8 Effects of Citicoline on Structural/Functional Consequences of Focal Ischemia of the Rat Brain]. Neurophysiology. 2022; 53, 78–87. doi.org/10.1007/s11062-022-09918-8
 +
 
 +
* Govbakh I, Kyryk V, Ustymenko A, Rubtsov V, Tsupykov O, Bulgakova NV, Zavodovskiy DO, Sokolowska I, Maznychenko A. [https://doi.org/10.3390/ijms222112026 Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy]. Int J Mol Sci. 2021 Nov 6;22(21):12026. doi.org/10.3390/ijms222112026
 +
 
 +
* Balatskyi VV, Vaskivskyi VO, Myronova A, Avramets D, Nahia KA, Macewicz LL, Ruban TP, Kucherenko DY, Soldatkin OO, Lushnikova IV, Skibo GG, Winata CL, Dobrzyn P, Piven OO. [https://doi.org/10.1016/j.mito.2021.07.005 Сardiac-specific β-catenin deletion dysregulates energetic metabolism and mitochondrial function in perinatal cardiomyocytes]. Mitochondrion. 2021 Sep;60:59-69. doi.org/10.1016/j.mito.2021.07.005
 +
 
 +
* Götz TWB, Puchkov D, Lysiuk V, Lützkendorf J, Nikonenko AG, Quentin C, Lehmann M, Sigrist SJ, Petzoldt AG. [https://doi.org/10.1083/jcb.202006040 Rab2 regulates presynaptic precursor vesicle biogenesis at the trans-Golgi]. J Cell Biol. 2021 May 3;220(5):e202006040.  doi.org/10.1083/jcb.202006040 
 +
 
 +
* Lushnikova I, Nikandrova Y, Skibo G. [https://doi.org/10.1007/s12264-021-00725-5 Mitochondrial Events Determine the Status of Hippocampal Cells in the Post-Ischemic Period]. Neurosci Bull. 2021 Aug;37(8):1246-1250. doi.org/10.1007/s12264-021-00725-5 
 +
 
 +
* Bozhok YM, Golovko O, Nikonenko AG. [https://doi.org/10.1016/j.cmpb.2020.105562 nPAsym: an open-source plugin for ImageJ to quantify nuclear shape asymmetry]. Comput Methods Programs Biomed. 2020;196:105562. doi.org/10.1016/j.cmpb.2020.105562
 +
 
 +
* Marungruang N, Kovalenko T, Osadchenko I, Voss U, Huang F, Burleigh S, Ushakova G, Skibo G, Nyman M, Prykhodko O, Hållenius FF. [https://doi.org/10.1080/1028415x.2018.1536423 Lingonberries and their two separated fractions differently alter the gut microbiota, improve metabolic functions, reduce gut inflammatory properties, and improve brain function in ApoE-/- mice fed high-fat diet]. Nutr Neurosci. 2020;23(8):600-612. doi.org/10.1080/1028415X.2018.1536423 
 +
 
 +
* Yatsenko K, Lushnikova I, Ustymenko A, Patseva M, Govbakh I, Kyryk V, Tsupykov O. [https://doi.org/10.3390/jpm10030066 Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice]. J Pers Med. 2020;10(3):66 doi.org/10.3390/jpm10030066
 +
 
 +
* Maznychenko AV, Bulgakova NV, Sokolowska IV, Butowska K, Borowik A, Mankivska OP, Piosik J, Tomiak T, Gonchar OO, Maisky VO, Kostyukov AI. [https://doi.org/10.1038/s41598-020-67034-1 Fatigue-induced Fos immunoreactivity within the lumbar cord and amygdala decreases after С60 fullerene pretreatment]. Sci Rep. 2020;10(1):9826. (https://www.nature.com/articles/s41598-020-67034-1
 +
 
 +
* Grønning Hansen M, Laterza C, Palma-Tortosa S, Kvist G, Monni E, Tsupykov O, Tornero D, Uoshima N, Soriano J, Bengzon J, Martino G, Skibo G, Lindvall O, Kokaia Z. [https://doi.org/10.1002/sctm.20-0134 Grafted human pluripotent stem cell-derived cortical neurons integrate into adult human cortical neural circuitry]. Stem Cells Transl Med. 2020;9(11):1365-1377. doi.org/10.1002/sctm.20-0134 
 +
 
 +
* Palma-Tortosa S, Tornero D, Grønning Hansen M, Monni E, Hajy M, Kartsivadze S, Aktay S, Tsupykov O, Parmar M, Deisseroth K, Skibo G, Lindvall O, Kokaia Z. [https://doi.org/10.1073/pnas.2000690117 Activity in grafted human iPS cell-derived cortical neurons integrated in stroke-injured rat brain regulates motor behavior]. Proc Natl Acad Sci U S A. 2020;117(16):9094-9100. doi.org/10.1073/pnas.2000690117 
  
−
The department organized a scientific base for students (undergraduate and masters) University Centre "Institute of Biology" KNU. Shevchenko Kyiv branch MIPT and Kyiv-Mohyla Academy.
+
* Kanemitsu M, Tsupykov O, Potter G, Boitard M, Salmon P, Zgraggen E, Gascon E, Skibo G, Dayer AG, Kiss JZ. [https://doi.org/10.1016/j.expneurol.2017.07.009 EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex]. Exp Neurol. 2017 Nov;297:14-24. doi.org/10.1016/j.expneurol.2017.07.009 
  
−
== Research activities ==
+
* Zoltowska KM, Maesako M, Lushnikova I, Takeda S, Keller LJ, Skibo G, Hyman BT, Berezovska O. [https://doi.org/10.1186/s13024-017-0159-y Dynamic presenilin 1 and synaptotagmin 1 interaction modulates exocytosis and amyloid β production]. Mol Neurodegener. 2017 Feb 13;12(1):15 dx.doi.org/10.1186%2Fs13024-017-0159-y
−
=== Main areas of research ===
+
−
* Study the viability and resistance hippocampal cells in modeling neyropatolohiy various origins (ischemia, stress, Alzheimer's disease) and search tools neuroprotection
+
−
* Study of pathophysiological mechanisms perinatal pathology of the central nervous system of different origins and opportunities uu therapeutic modulation
+
−
* Study the cellular mechanisms of neurodegeneration in the early stages of Parkinson's disease model rotenonoviy
+
−
* Depending on the study of structural and functional features of the brain from the gastrointestinal tract.
+
−
* Research features regenerative stem cells in experimentally induced neurodegenerative pathologies of the brain (ischemia, brain injury)
+
  
−
=== Research objects ===
+
* Tornero D, Tsupykov O, Granmo M, Rodriguez C, Grønning-Hansen M, Thelin J, Smozhanik E, Laterza C, Wattananit S, Ge R, Tatarishvili J, Grealish S, Brüstle O, Skibo G, Parmar M, Schouenborg J, Lindvall O, Kokaia Z. [https://doi.org/10.1093/brain/aww347 Synaptic inputs from stroke-injured brain to grafted human stem cell-derived neurons activated by sensory stimuli]. Brain. 2017 Mar 1;140(3):692-706. /doi.org/10.1093/brain/aww347 
−
* Animals (rats, mice, gerbils, pigs)
+
−
* Cell culture (orhanotypovi and dissociated culture)
+
  
−
=== Research Methods ===
+
* Gerth F, Pechstein A, Kochlamazashvili G, Jäpel M, Lehmann M, Puchkov D, Onofri F, Benfenati F, Nikonenko AG, Maritzen T, Freund Ch, Haucke V. [https://doi.org/10.1073/pnas.1715341114 Intersectin associates with synapsin and regulates its nanoscale localization and function]. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12057-12062. dx.doi.org/10.1073%2Fpnas.1715341114 
−
* Modeling neurodegenerative conditions in vivo (cerebral ischemia, Parkinson's disease, perinatal pathology of the central nervous system, brain injury, exocrine pancreatic insufficiency) and in vitro (cerebral ischemia, Alzheimer's disease, stress);
+
−
* Microscopy: light, confocal, electron,
+
−
* Immunohistochemistry methods,
+
−
* Reverse transcription and polymerase chain reaction (single-cell RT-PCR),
+
−
* Imunoblotinh,
+
−
* Quantitative ultrastructural analysis
+
−
* Computerized image analysis,
+
−
* Morphometry.  
+
−
       
+
−
== List of laboratories with which there is scientific cooperation ==
+
−
* Department of Cell Biology and body Lund University, Sweden
+
−
* Institute of Neurobiology of the Slovak Academy of Sciences, Kosice, Slovakia
+
−
* Institute of brain dynamics National Institute for Health and Medical Research (INSERM), Marseille, France
+
−
* Max Delbruk Center for Molecular Medicine, Neuroscience Group, Berlin-Buch, Germany
+
  
−
== Scientific advances. Selected publications for years 2011-2014 ==
+
* Kopach O, Maistrenko A, Lushnikova I, Belan P, Skibo G, Voitenko N. [https://doi.org/10.1016/j.ceca.2016.02.014 HIF-1α-mediated upregulation of SERCA2b: The endogenous mechanism for alleviating the ischemia-induced intracellular Ca(2+) store dysfunction in CA1 and CA3 hippocampal neurons]. Cell Calcium. 2016. May;59(5):251-61. doi.org/10.1016/j.ceca.2016.02.014   
  
−
# Tsupykov O, Kyryk V, Smozhanik E, Rybachuk O, Butenko G, Pivneva T, Skibo G.  Long-term fate of grafted hippocampal neural progenitor cells following ischemic injury. J Neurosci Res. 2014 Apr 22.
+
* Tsupykov O, Kanemitsu M, Smozhanik E, Skibo G, Dayer AG, Kiss JZ. [https://doi.org/10.3727/096368916x690421 Relationship of Grafted FGF-2-Overexpressing Neural Stem/Progenitor Cells With the Vasculature in the Cerebral Cortex]. Cell Transplant. 2016;25(7):1359-69. doi.org/10.3727%2F096368916X690421
−
# Rybachuk O. A., Kyryk V. M, Poberezhnyi P. A., Butenko G. M., Skibo G. G., Pivneva T. A. Effect of bone marrow multipotent mesenchymal stromal cells on the neural tissue after ischemic injury in vitro, Cell and organ transplantology, - 2014, 2, № 1, p. 96-100.
+
−
# Pierzynowski S, Ushakova G, Kovalenko T, Osadchenko I, Goncharova K, Gustavsson P, Prykhodko O, Wolinski J, Slupecka M, Ochniewicz P, Weström B, Skibo G. Impact of colostrum and plasma immunoglobulin intake on hippocampus structure during early postnatal development in pigs. Int J Dev Neurosci. 2014 Mar 15. pii: S0736-5748(14)00037-9.
+
−
# Nikonenko I, Nikonenko A, Mendez P, Michurina TV, Enikolopov G, Muller D. Nitric oxide mediates local activity-dependent excitatory synapse development. Proc Natl Acad Sci U S A. 2013, 110(44):E4142-4151.
+
−
# Rybachuk О. А, Levin R. E., Кyryk V. М., Susarova D. K., Tsupykov О. M., Smozhanik E. G., Butenko G. M., Skibo G. G., Troshin P. A., Pivneva Т. А. Effect of a water soluble derivative of fullerene C60 on the features neural progenitor cells in vitro, Cell and organ transplantology, 2013, 1, № 1, 96-107
+
−
# Никоненко А.Г. Введение в количественную гистологию. Киев: Книга плюс, 2013, 256с. 500 прим. 15,5 друк. арк.
+
−
# Tress O, Maglione M, May D, Pivneva T, Richter N, Seyfarth J, Binder S, Zlomuzica A, Seifert G, Theis M, Dere E, Kettenmann H, Willecke K. Panglial gap junctional communication is essential for maintenance of myelin in the CNS. J. Neurosci.  2012.  32(22).  P.7499-518.
+
−
# Pierzynowski S, Swieboda P, Filip R, Szwiec K, Valverde Piedra JL, Gruijc D, Prykhodko O, Fedkiv O, Kruszewska D, Botermans J, Svendsen J, Skibo G, Kovalenko T, Osadchenko I, Goncharova K, Ushakova G, Weström B. Behavioral changes in response to feeding pancreatic-like enzymes to exocrine pancreatic insufficiency pigs. J Anim Sci. 2012 Dec;90 Suppl 4:439-41.
+
−
# Woliński J, Słupecka M, Weström B, Prykhodko O, Ochniewicz P, Arciszewski M, Ekblad E, Szwiec K, Ushakova, Skibo G, Kovalenko T, Osadchenko I, Goncharova K, Botermans J, Pierzynowski S. Effect of feeding colostrum versus exogenous immunoglobulin G on gastrointestinal structure and enteric nervous system in newborn pigs. J Anim Sci. 2012 Dec;90 Suppl 4:327-30.
+
−
# Pierzynowski S, Szwiec K, Valverde Piedra JL, Gruijc D, Szymanczyk S, Swieboda P, Prykhodko O, Fedkiv O, Kruszewska D, Filip R, Botermans J, Svendsen J, Ushakova G, Kovalenko T, Osadchenko I, Goncharova K, Skibo G, Weström B. Exogenous pancreatic-like enzymes are recovered in the gut and improve growth of exocrine pancreatic insufficient pigs. J Anim Sci. 2012 Dec;90 Suppl 4:324-6.
+
−
# Kopach O, Kruglikov I, Pivneva T, Voitenko N, Verkhratsky A, Fedirko N. Mitochondria adjust Ca(2+) signaling regime to a pattern of stimulation in salivary acinar cells. Biochim Biophys Acta. 2011 Oct;1813(10):1740-8.
+
−
# Puchkov D, Leshchyns'ka I, Nikonenko AG, Schachner M, Sytnyk V. NCAM/spectrin complex disassembly results in PSD perforation and postsynaptic endocytic zone formation. Cereb Cortex. 2011 Oct;21(10):2217-32.
+
−
# Tsupykov O.M., Poddubnaya A.O., Smozhanyk K.G., Kyryk V.M., Kuchuk O.V., Butenko G.M.,  Semenova E.A., Pivneva T.A., Skibo G.G. Integration of grafted neural progenitor cells in host hippocampal circuitry after ischemic injury// Neurophysiology. –  2011. –  V.43., №.4., P. 372-375.
+
−
# Lushnikova I, Skibo G, Muller D, Nikonenko I. Excitatory synaptic activity is associated with a rapid structural plasticity of inhibitory synapses on hippocampal CA1 pyramidal cells. Neuropharmacology. 2011 Apr;60(5):757-64.
+
−
# Lushnikova I, Orlovsky M, Dosenko V, Maistrenko A, Skibo G. Brief anoxia preconditioning and HIF prolyl-hydroxylase inhibition enhances neuronal resistance in organotypic hippocampal slices on model of ischemic damage. Brain Res. 2011 Apr 22;1386:175-83.
+

Latest revision as of 08:24, 27 June 2022

Department of Cytology has been established in 1996 based on the Laboratory of Neurocytology. Its organizer and the only chair until nowadays is [G.G.Skibo|Prof. Galyna Skibo], the corresponding member of NAS of Ukraine, laureate of State Prize of Ukraine in sciences and engineering, Bogomoletz and Kostiuk Prizes of NAS of Ukraine.

Contents

Our research

The research activity of the Department of Cytology is focused on the study of cellular and molecular mechanisms of neurodegeneration in different parts of the nervous system and the search for neuroprotective approaches. The current research topics of the Department are:

  • genetically determined molecular mechanisms of intercellular and intracellular signaling in norm and pathology in vitro (brain ischemia, Alzheimer’s disease)
  • molecular and cellular mechanisms of endogenous neuroprotection in the models of neurodegenerative diseases in vivo and in vitro (brain ischemia)
  • regenerative properties of different origin stem cells and peculiarities of their application at experimentally induced neurodegenerative pathologies in vivo (brain ischemia, perinatal CNS pathology, neuroinflammation)
  • development of biosafety criteria for stem cell cultivation
  • cell mechanisms driving brain tissue degeneration in the rotenone model of Parkinson's disease
  • dependence of structural and functional features of the brain on the function of the gastrointestinal tract (metabolic syndrome, exocrine pancreatic insufficiency) in vivo and in vitro
  • effect of substances with antioxidant properties on the nervous tissue recovery of experimental animals during the brain ischemia-reperfusion


  • Synapses with continuous (A) and perforated (B) postsynaptic densities (red lines) between host dendritic spines (ds, brown) and grafted GFP+/DAB+ presynaptic axon terminals (at, green) in the contralateral somatosensory cortex of stroke-injured rats. (Scale bar – 0.2 μm) Source
  • The GIF animation created using serial electron micrographs of the hippocampal CA1 area, illustrating the direct interaction of the pyramidal neuron and astrocyte by exocytosis/endocytosis (to see that animation, open full image) Source
  • FGF-2-Overexpressing Neural Stem/Progenitor Cells (FGF-2-NPCs) made contact with blood vessels at sites with astrocyte coverage, but no pericytes Source
  • Synapses in СА1 stratum radiatum in vivo Red arrow-simple, yellow-multiple, green-perforated Source
  • 3D reconstructions of spine synapses contacted by glia under ischemic conditions Source

Research objects

  • Animals: rats, mice, gerbils, pigs;
  • Cell cultures: organotypic cultures of the hippocampus, dissociated cultures of CNS neurons, enteric neuron cultures.

Research methods

  • modelling of neurodegenerative states in vivo (cerebral ischemia, Parkinson’s disease, neuroinflammation, perinatal pathology of CNS, metabolic syndrome, exocrine pancreatic insufficiency) and in vitro (cerebral ischemia, Alzheimer’s disease, stress);
  • light, confocal, and transmission electron microscopy;
  • immunohistochemistry;
  • behavioral tests (open field, balance beam walking, Morris water maze, T- maze)
  • immunoblotting;
  • morphometry: computerized image analysis, quantitative ultrastructural analysis.

Collaboration

  • Lund Stem Cell Center, Lund University, Lund, Sweden;
  • Food for Health Science Center, Lund University, Lund, Sweden;
  • Chebotarev State Institute of Gerontology NAMS of Ukraine, Kyiv, Ukraine;
  • Oles Honchar Dnipro National University, Dnipro, Ukraine.

Team

  1. Galyna Skibo – Prof., DSc, corresponding member of NAS of Ukraine, head of Department, skibo@biph.kiev.ua
  2. Oleksandr Nikonenko – DSc, leading researcher, agn@biph.kiev.ua
  3. Oleh Tsupykov – DSc, leading researcher, tsupykov@gmail.com
  4. Iryna Lushnikova – DSc, leading researcher, li@biph.kiev.ua
  5. Tetiana Kovalenko – Candidate of sciences (PhD), senior researcher, tnk@biph.kiev.ua
  6. Iryna Osadchenko – Candidate of sciences (PhD), senior researcher, osad@biph.kiev.ua
  7. Olena Mankivska – Candidate of sciences (PhD), senior researcher emankovskaya@biph.kiev.ua
  8. Kateryna Yatsenko – DSc, senior researcher, kateryna.yatsenko@gmail.com
  9. Maryna Patseva – Candidate of sciences (PhD), researcher, pma@biph.kiev.ua
  10. Olena Savchuk – Candidate of sciences (PhD), junior researcher, floweringbowl@ukr.net
  11. Kateryna Smozhanyk – technician.
  12. Petro Maiorenko – technician.
  13. Liudmyla Melnychuk - laboratory assistant.

PhD students

  1. Nataliia Chaika - nataliia_chaika@ukr.net
  2. Olha Kostiuchenko - kostiuchenko.olha@biph.kiev.ua
  3. Nadiia Kravchenko - kravchenko.nadiia@biph.kiev.ua
  4. Dmytro Shepilov - shepilov@biph.kiev.ua

Recent publications

Retrieved from "http://biph.kiev.ua/en?title=Department_of_Cytology&oldid=14933"
Personal tools
Navigation
societies
additional
Views
Namespaces
Variants
Toolbox
Actions