NMP:Methods

Bogomoletz Institute of Physiology
Jump to: navigation, search

Methods

Scientific
methods

How we do our research

The main objects and suppliers of biological materials for our research are laboratory rats.
Many thanks to these small animals for their invaluable contribution to the world's science

Lab_rat

Animal (rat) models of pathologies:

  • Streptozotocin(STZ)-induced diabetes;
  • Cyclophosphamide(CPA)-induced cystitis;
  • N-butyl-N-(4-hydroxybutyl)nitrosamine(BBN)-induced bladder cancer;
  • WAG/Rij model of absence epilepsy.
Cells

Primary cells and cell lines:

  • Acutely isolated and cultured primary cell from various rat tissues (neurons, smooth muscle cells, cardiac myocytes, epithelial cells, etc.;
  • Cellular systems for heterologous expression of foreign channels and receptors (Xenopus oocytes, cultured HEK-293 cells);
  • Cell lines (LNCaP human prostate cancer cell line, P19 pluripotent mouse embryonal carcinoma cells).
PatchClamp

Electrophysiology, tensiometry:

  • Patch-clamp;
  • Double microelectrode voltage-clamp;
  • Muscle contraction force measurements.
Confocal

Microscopy:

  • Transmitted light microscopy;
  • Fluorescence microscopy;
  • Confocal laser-scanning microscopy;
Molecular_Biology

Molecular biology:

  • Heterologous expression;
  • Cells transformation;
  • mRNA isolation;
  • Polymerase chain reaction (PCR).
Blotter_paper

Immunochemistry:

  • Western blot;
  • Fluorescence immunocytochemistry;
Personal tools
Navigation
societies
additional
Views
Namespaces
Variants
Toolbox
Actions