DHSI:Methods
Інститут фізіології ім. О. О. Богомольця
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<br>'''Our methods''' | <br>'''Our methods''' | ||
<br>'''Animal (rat) models of pathologies:''' | <br>'''Animal (rat) models of pathologies:''' | ||
| + | [[File:animal models.jpg|200px|thumb|Animal models|right]] | ||
| + | |||
*Rotenone Parkinson desease model; | *Rotenone Parkinson desease model; | ||
*Streptozotocin(STZ)-induced diabetes; | *Streptozotocin(STZ)-induced diabetes; | ||
*Doxorubicin-indused oxidative stress; | *Doxorubicin-indused oxidative stress; | ||
*Anoxia-reoxigenation oxidative stress; | *Anoxia-reoxigenation oxidative stress; | ||
| − | * | + | *Culture of neonatal cardiomyocytes (in collaboration). |
'''In our Department we do:''' | '''In our Department we do:''' | ||
'''Microscopy:''' | '''Microscopy:''' | ||
| + | <br>[[File:Microscopy.jpg|200px|thumb|Microscopy|right]] | ||
*Transmitted light microscopy; | *Transmitted light microscopy; | ||
'''Immunochemistry:''' | '''Immunochemistry:''' | ||
*Western blot; | *Western blot; | ||
| + | <br>[[File:Western blot.jpg|200px|thumb|Western blot|right]] | ||
'''Biochemical:''' | '''Biochemical:''' | ||
*Determination of the oxidative and nitrosatitative stresses - hydrogen peroxide, superoxide radical, hydroxyl radical, diene conjugates, malonic dialdehyde in homogenates of the heart, aorta, medulla oblongata and suspension of mitochondria; | *Determination of the oxidative and nitrosatitative stresses - hydrogen peroxide, superoxide radical, hydroxyl radical, diene conjugates, malonic dialdehyde in homogenates of the heart, aorta, medulla oblongata and suspension of mitochondria; | ||
*Determination of the antioxidant system activity (SODs, catalase, GSH-Px) | *Determination of the antioxidant system activity (SODs, catalase, GSH-Px) | ||
*Polarographic investigation of tissue PO2 and mitochondrial respiration | *Polarographic investigation of tissue PO2 and mitochondrial respiration | ||
| + | {| class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | !width=600|[[File:DHSI_1.jpg|600px]] | ||
| + | |-style="background:#ccffcc;" | ||
| + | |style="text-align:left;"|'''Polarographic investigation of tissue PO2 and mitochondrial respiration (V. Nosar, L. Bratus)''' | ||
| + | |} | ||
| + | {| class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | !width=600|[[File:DHSI_5.jpg|600px]] | ||
| + | |-style="background:#ccffcc;" | ||
| + | |style="text-align:left;"|'''The original set for studying of the pattern of breathing at small laboratory animals<br> (V. Portnichenko, A. Sidorenko, M. Kolcheva)''' | ||
| + | |} | ||
'''Studying of the pattern of breathing at small laboratory animals''' | '''Studying of the pattern of breathing at small laboratory animals''' | ||
'''Methods of pathological conditions correction:''' | '''Methods of pathological conditions correction:''' | ||
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*Pharmacological – flavonoids, drugs, etc. | *Pharmacological – flavonoids, drugs, etc. | ||
'''Medico-biological investigations at the Ukrainian Antarctic Station Academician Vernadsky.''' | '''Medico-biological investigations at the Ukrainian Antarctic Station Academician Vernadsky.''' | ||
| − | '''In collaboration with other Departments:''' | + | {| class="wikitable" style="text-align: center;" |
| − | '''Microscopy:''' | + | |- |
| + | !width=600|[[File:DHSI_2.jpg|600px]] | ||
| + | |-style="background:#ccffcc;" | ||
| + | |style="text-align:left;"|'''Medico-biological investigations at the <br>Ukrainian Antarctic Station Academician Vernadsky (E. Moiseyenko)''' | ||
| + | |} | ||
| + | |||
| + | {| class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | !width=600|[[File:DHSI_3.jpg|600px]] | ||
| + | |-style="background:#ccffcc;" | ||
| + | |style="text-align:left;"|'''Elbrus Medical and Biological Station of the International Center <br>of astronomical and medical and environmental research of NAS of Ukraine<br> at a height of 2100m in the Elbrus region.''' | ||
| + | |} | ||
| + | |||
| + | <br>'''In collaboration with other Departments:''' | ||
| + | <br>'''Microscopy:''' | ||
| + | <br>[[File:animal models1.jpg|200px|thumb|Neonatal cardiomyocytes culture|right]] | ||
*Fluorescence microscopy; | *Fluorescence microscopy; | ||
*Confocal laser-scanning microscopy; | *Confocal laser-scanning microscopy; | ||
| + | |||
'''Molecular biology:''' | '''Molecular biology:''' | ||
| + | <br>[[File:RT-PCR.jpg|200px|thumb|RT-PCR|right]] | ||
*Heterologous expression; | *Heterologous expression; | ||
*Cells transformation; | *Cells transformation; | ||
*mRNA isolation; | *mRNA isolation; | ||
*Polymerase chain reaction (PCR) | *Polymerase chain reaction (PCR) | ||
Latest revision as of 14:27, 11 May 2018
In our Department we do: Microscopy: 
Immunochemistry:
 Biochemical:
Studying of the pattern of breathing at small laboratory animals Methods of pathological conditions correction:
Medico-biological investigations at the Ukrainian Antarctic Station Academician Vernadsky.
Molecular biology: 
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