Name: Olga Gonchar, Ph.D.
Degree: Ph D, Biology (Specialization Physiology of Human and Animals)
Bogomolets Institute of Physiology National Academy of Sciences of Ukraine, 1996
M.Sc., with honour, Biology and Chemistry
Taras Shevchenko Pedagogical University of Lugansk, 1984
Present position:
Senior researcher, Department of Hypoxic States, Bogomolets Institute of Physiology National Academy of Sciences of Ukraine
Academic and professional honours and awards:
Grand of the Forchungsgruppe “Neurohormonale Wirkungsmechanismen” der Sachsischen Academie der Wissenchaften zu Leipzig an der Friedrich-Schiller-Universitat, Jena, 2002.
Member of Ukrainian Physiological Society, Member of Ukrainian Pathophysiological Society
Field of research:
biochemistry, physiology, pathophysiology, cellular and molecular biology
Practical Experience: Research of molecular and genetic mechanisms regulation of prooxidant-antioxidant homeostasis, energy metabolism and apoptosis in cells during the development of oxidative stress different genesis (immobilization, hypoxia of various origin, and cardio- and neurotoxins) as well as the role of intracellular thiols (glutathione and thioredoxin systems) and antioxidant enzymes (MnSOD, catalase, etc.) in cellular redox-potential shaping and redox-signaling implementation. Study the peculiarities of compensatory-adaptive reactions formation involving redox-sensitive nuclear transcription factors under the various modes of interval hypoxic training, hypoxic and hypoxic-hyperoxic preconditioning, restrain stress. The correction of pathophysiological disorders by new pharmacological substances.
Procedures and skills:
-Animal handling experience (different models of intermittent hypoxic training and hypoxic states of different genesis, stress exposures, physical exercises, drug intoxication in rats);
- Isolation of the subcellular fractions of rats tissues (cytosol, mitochondria, lysosome, nucleus) and cells of blood;
- Assay of the content of lipid peroxidation products, activity of antioxidant enzymes, enzymatic complexes of the respiratory chain in mitochondria and enzymes of different metabolic cycles;
- determination of the glutathion and thioredoxin systems components (enzymatic and nonenzymatic);
- western transfer and immunoblotting;
- electrophoresis;
- RNA isolation
Key publications
- O Gonchar, I Mankovska.Moderate hypoxia/hyperoxia attenuates acute hypoxia-induced oxidative damage and improves antioxidant defense in lung mitochondria. Acta Physiol Hung. 2012, 99 (4), 436-446
- O Gonchar, I Mankovska. Antioxidant system in adaptation to intermittent hypoxia. J. Biol. Sci.2010, 10 (6), 545-554
- O Gonchar. Muscle fiber specific antioxidative system adaptation to swim training in rats: influence of intermittent hypoxia. J Sports Sci Med. 2005, 4 (2), 160-169.
- O Gonchar, I Mankovska. Effect of moderate hypoxia/reoxygenation on mitochondrial adaptation to acute severe hypoxia. Acta Biologica Hung. 2009, 60 (2), 185-194
- O Gonchar, I Mankovska. Glutathione system adaptation to acute stress in the heart of rats during different regimes of hypoxia training. Ukr. Biochem. J.1999, 79 (3), 79-85
- ОА Гончар, ЕВ Розова. Влияние разных режимов интервальных гипоксических тренировок на морфологические характеристики и антиоксидантный статус тканей сердца и легких.Бюл. Экспер. Биол. и Мед. 2007, 144 (8), 216-220
- YI Prylutskyy, IV Vereshchaka, AV Maznychenko, NV Bulgakova, O O Gonchar, OA Kyzyma, U Ritter, P Scharff, T Tomiak, D M Nozdrenko, I V Mishchenko, AI Kostyukov. C 60 fullerene as promising therapeutic agent for correcting and preventing skeletal muscle fatigue. J of nanobiotechnology . 2017, 15 (1), 1-8
- O Gonchar, I Mankovska. Hypoxia/Reoxygenation modulates Oxidative Stress Level and Antioxidative Potential in Lung Mitochondria: Possible participation of P53 and NF-KB Target Proteins. Arch Pulmonol Respir Care, 2017, 3(2):035-043
- O Gonchar, I Mankovska.Time-dependent effect of severe hypoxia/reoxygenation on oxidative stress level, antioxidant capacity and p53 accumulation in heart mitochondria. Ukr. Biochem. J. 2017, 89(6): 41-49
- Mankovska I. and Gonchar O. Response of mitochondrial thiol-redox systems to an oxidant challenge caused by acute hypoxia: protective effect of adaptation to hypoxia/hyperoxia. In: Adaptation Biology and Medicine. Eds. Laurentiu M. Popescu, A.R. Hargens and Pawan K Singal, Narosa Publishing House Pvt. Ltd., New Delhi, India, 2013, v.7, p. 169-181.
Contact
Bogomoletz Str, 01024 Kyiv, Ukraine
Department of Hypoxic States (office322)
e-mail: olga.gonchar@i.ua
Phone +38044 256 24 92
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