Nuclear shape software

A Psymmetry 2.1 software allows to estimate nuclear shape asymmetry in digital images using two scenarios. Within the first one, nuclear pixels laying outside the largest inscribed circle are identified. According to the second scenario, the algorithm searches for nuclear pixels lacking pixel-partners symmetric with respect to the nuclear area’s centroid. In both cases the proportion of ‘asymmetric’ pixels is used to estimate the feature of interest. The technique was validated on images of cell nuclei having distinctive shape phenotypes.

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