Department of Cytology
Інститут фізіології ім. О. О. Богомольця
Версія від 14:05, 22 червня 2022; Andrii.cherninskyi (Обговорення • внесок)
Department of Cytology has been established in 1996 based on the Laboratory of Neurocytology. Its organizer and the only chair until nowadays is [G.G.Skibo|Prof. Galyna Skibo], the corresponding member of NAS of Ukraine, laureate of State Prize of Ukraine in sciences and engineering, Bogomoletz and Kostiuk Prizes of NAS of Ukraine.
Зміст |
Our research
The research activity of the Department of Cytology is focused on the study of cellular and molecular mechanisms of neurodegeneration in different parts of the nervous system and the search for neuroprotective approaches. The current research topics of the Department are:
- genetically determined molecular mechanisms of intercellular and intracellular signaling in norm and pathology in vitro (brain ischemia, Alzheimer’s disease)
- molecular and cellular mechanisms of endogenous neuroprotection in the models of neurodegenerative diseases in vivo and in vitro (brain ischemia)
- regenerative properties of different origin stem cells and peculiarities of their application at experimentally induced neurodegenerative pathologies in vivo (brain ischemia, perinatal CNS pathology, neuroinflammation)
- development of biosafety criteria for stem cell cultivation
- cell mechanisms driving brain tissue degeneration in the rotenone model of Parkinson's disease
- dependence of structural and functional features of the brain on the function of the gastrointestinal tract (metabolic syndrome, exocrine pancreatic insufficiency) in vivo and in vitro
- effect of substances with antioxidant properties on the nervous tissue recovery of experimental animals during the brain ischemia-reperfusion
Research objects
- Animals: rats, mice, gerbils, pigs;
- Cell cultures: organotypic cultures of the hippocampus, dissociated cultures of CNS neurons, enteric neuron cultures.
Research methods
- modelling of neurodegenerative states in vivo (cerebral ischemia, Parkinson’s disease, neuroinflammation, perinatal pathology of CNS, metabolic syndrome, exocrine pancreatic insufficiency) and in vitro (cerebral ischemia, Alzheimer’s disease, stress);
- light, confocal, and transmission electron microscopy;
- immunohistochemistry;
- behavioral tests (open field, balance beam walking, Morris water maze, T- maze)
- immunoblotting;
- morphometry: computerized image analysis, quantitative ultrastructural analysis.
Collaboration
- Lund Stem Cell Center, Lund University, Lund, Sweden;
- Food for Health Science Center, Lund University, Lund, Sweden;
- Chebotarev State Institute of Gerontology NAMS of Ukraine, Kyiv, Ukraine;
- Oles Honchar Dnipro National University, Dnipro, Ukraine.
Team
- Galyna Skibo – Prof., DSc, corresponding member of NAS of Ukraine, head of Department, skibo@biph.kiev.ua
- Oleksandr Nikonenko – DSc, leading researcher, agn@biph.kiev.ua
- Oleh Tsupykov – DSc, leading researcher, tsupykov@gmail.com
- Iryna Lushnikova – DSc, leading researcher, li@biph.kiev.ua
- Tetiana Kovalenko – Candidate of sciences (PhD), senior researcher, tnk@biph.kiev.ua
- Iryna Osadchenko – Candidate of sciences (PhD), senior researcher, osad@biph.kiev.ua
- Olena Mankivska – Candidate of sciences (PhD), senior researcher emankovskaya@biph.kiev.ua
- Kateryna Yatsenko – DSc, senior researcher, kateryna.yatsenko@gmail.com
- Maryna Patseva – Candidate of sciences (PhD), researcher, pma@biph.kiev.ua
- Olena Savchuk – Candidate of sciences (PhD), junior researcher, floweringbowl@ukr.net
- Kateryna Smozhanyk – technician.
- Petro Maiorenko – technician.
- Liudmyla Melnychuk - laboratory assistant.
PhD students
- Nataliia Chaika - nataliia_chaika@ukr.net
- Olha Kostiuchenko - kostiuchenko.olha@biph.kiev.ua
- Nadiia Kravchenko - kravchenko.nadiia@biph.kiev.ua
- Dmytro Shepilov - shepilov@biph.kiev.ua
Recent publications
- Shepilov D, Kovalenko T, Osadchenko I, Smozhanyk K, Marungruang N, Ushakova G, Muraviova D, Hållenius F, Prykhodko O, Skibo G. Varying Dietary Component Ratios and Lingonberry Supplementation May Affect the Hippocampal Structure of ApoE-/- Mice. Front Nutr. 2022 Feb 16;9:565051. doi.org/10.3389/fnut.2022.565051
- Mankivska, O.P., Chaika, N.V. & Skibo, G.G. Effects of Citicoline on Structural/Functional Consequences of Focal Ischemia of the Rat Brain. Neurophysiology. 2022; 53, 78–87. doi.org/10.1007/s11062-022-09918-8
- Govbakh I, Kyryk V, Ustymenko A, Rubtsov V, Tsupykov O, Bulgakova NV, Zavodovskiy DO, Sokolowska I, Maznychenko A. Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy. Int J Mol Sci. 2021 Nov 6;22(21):12026. doi.org/10.3390/ijms222112026
- Balatskyi VV, Vaskivskyi VO, Myronova A, Avramets D, Nahia KA, Macewicz LL, Ruban TP, Kucherenko DY, Soldatkin OO, Lushnikova IV, Skibo GG, Winata CL, Dobrzyn P, Piven OO. Сardiac-specific β-catenin deletion dysregulates energetic metabolism and mitochondrial function in perinatal cardiomyocytes. Mitochondrion. 2021 Sep;60:59-69. doi.org/10.1016/j.mito.2021.07.005
- Götz TWB, Puchkov D, Lysiuk V, Lützkendorf J, Nikonenko AG, Quentin C, Lehmann M, Sigrist SJ, Petzoldt AG. Rab2 regulates presynaptic precursor vesicle biogenesis at the trans-Golgi. J Cell Biol. 2021 May 3;220(5):e202006040. doi.org/10.1083/jcb.202006040
- Lushnikova I, Nikandrova Y, Skibo G. Mitochondrial Events Determine the Status of Hippocampal Cells in the Post-Ischemic Period. Neurosci Bull. 2021 Aug;37(8):1246-1250. doi.org/10.1007/s12264-021-00725-5
- Bozhok YM, Golovko O, Nikonenko AG. nPAsym: an open-source plugin for ImageJ to quantify nuclear shape asymmetry. Comput Methods Programs Biomed. 2020;196:105562. doi.org/10.1016/j.cmpb.2020.105562
- Marungruang N, Kovalenko T, Osadchenko I, Voss U, Huang F, Burleigh S, Ushakova G, Skibo G, Nyman M, Prykhodko O, Hållenius FF. Lingonberries and their two separated fractions differently alter the gut microbiota, improve metabolic functions, reduce gut inflammatory properties, and improve brain function in ApoE-/- mice fed high-fat diet. Nutr Neurosci. 2020;23(8):600-612. doi.org/10.1080/1028415X.2018.1536423
- Yatsenko K, Lushnikova I, Ustymenko A, Patseva M, Govbakh I, Kyryk V, Tsupykov O. Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice. J Pers Med. 2020;10(3):66 doi.org/10.3390/jpm10030066
- Maznychenko AV, Bulgakova NV, Sokolowska IV, Butowska K, Borowik A, Mankivska OP, Piosik J, Tomiak T, Gonchar OO, Maisky VO, Kostyukov AI. Fatigue-induced Fos immunoreactivity within the lumbar cord and amygdala decreases after С60 fullerene pretreatment. Sci Rep. 2020;10(1):9826. (https://www.nature.com/articles/s41598-020-67034-1
- Grønning Hansen M, Laterza C, Palma-Tortosa S, Kvist G, Monni E, Tsupykov O, Tornero D, Uoshima N, Soriano J, Bengzon J, Martino G, Skibo G, Lindvall O, Kokaia Z. Grafted human pluripotent stem cell-derived cortical neurons integrate into adult human cortical neural circuitry. Stem Cells Transl Med. 2020;9(11):1365-1377. doi.org/10.1002/sctm.20-0134
- Palma-Tortosa S, Tornero D, Grønning Hansen M, Monni E, Hajy M, Kartsivadze S, Aktay S, Tsupykov O, Parmar M, Deisseroth K, Skibo G, Lindvall O, Kokaia Z. Activity in grafted human iPS cell-derived cortical neurons integrated in stroke-injured rat brain regulates motor behavior. Proc Natl Acad Sci U S A. 2020;117(16):9094-9100. doi.org/10.1073/pnas.2000690117
- Kanemitsu M, Tsupykov O, Potter G, Boitard M, Salmon P, Zgraggen E, Gascon E, Skibo G, Dayer AG, Kiss JZ. EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex. Exp Neurol. 2017 Nov;297:14-24. doi.org/10.1016/j.expneurol.2017.07.009
- Zoltowska KM, Maesako M, Lushnikova I, Takeda S, Keller LJ, Skibo G, Hyman BT, Berezovska O. Dynamic presenilin 1 and synaptotagmin 1 interaction modulates exocytosis and amyloid β production. Mol Neurodegener. 2017 Feb 13;12(1):15 dx.doi.org/10.1186%2Fs13024-017-0159-y
- Tornero D, Tsupykov O, Granmo M, Rodriguez C, Grønning-Hansen M, Thelin J, Smozhanik E, Laterza C, Wattananit S, Ge R, Tatarishvili J, Grealish S, Brüstle O, Skibo G, Parmar M, Schouenborg J, Lindvall O, Kokaia Z. Synaptic inputs from stroke-injured brain to grafted human stem cell-derived neurons activated by sensory stimuli. Brain. 2017 Mar 1;140(3):692-706. /doi.org/10.1093/brain/aww347
- Gerth F, Pechstein A, Kochlamazashvili G, Jäpel M, Lehmann M, Puchkov D, Onofri F, Benfenati F, Nikonenko AG, Maritzen T, Freund Ch, Haucke V. Intersectin associates with synapsin and regulates its nanoscale localization and function. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12057-12062. dx.doi.org/10.1073%2Fpnas.1715341114
- Kopach O, Maistrenko A, Lushnikova I, Belan P, Skibo G, Voitenko N. HIF-1α-mediated upregulation of SERCA2b: The endogenous mechanism for alleviating the ischemia-induced intracellular Ca(2+) store dysfunction in CA1 and CA3 hippocampal neurons. Cell Calcium. 2016. May;59(5):251-61. doi.org/10.1016/j.ceca.2016.02.014
- Tsupykov O, Kanemitsu M, Smozhanik E, Skibo G, Dayer AG, Kiss JZ. Relationship of Grafted FGF-2-Overexpressing Neural Stem/Progenitor Cells With the Vasculature in the Cerebral Cortex. Cell Transplant. 2016;25(7):1359-69. doi.org/10.3727%2F096368916X690421