Department of Neuronal Networks Physiology:3
Methods
Low-density culture of dissociated neurons from peripheral and central nervous system regions of rats. The primary cultures of dorsal root ganglia, upper cervical ganglia, and trigeminal ganglia neurons (Telka, Rikhalsky, Veselovsky, 2019).
Coculture of dissociated retinal ganglion cells and superficial superior colliculus (SSC) neurons from Wistar rats. Coculture of dissociated dorsal root ganglion (DRG) neurons and dorsal horn (DH) spinal cord neurons with the formation of functional synaptic connections.
Electrophysiological studies of synaptic transmission using the patch-clamp technique in the whole-cell configuration in voltage- or current-clamp modes, employing extracellular stimulation of a single presynaptic neuron axon or an individual presynaptic terminal.
Electrophysiological studies of synaptic transmission using paired recordings in two synaptically connected neurons simultaneously, by stimulating the soma of the presynaptic neuron and simultaneously recording postsynaptic currents in the postsynaptic neuron (Pfrieger, Veselovsky et al., 1992)
Fast local superfusion (Veselovsky et al., 1996) and fast-flow perfusion methods for in vitro application of pharmacological agents during electrophysiological recordings.
Application of in vitro experimental models of pathological conditions in electrophysiological studies: artificial hypoinsulinemia, hyperglycemia, and hypoxia according to previously described methods (Dumanska&Veselovsky, 2019; Shypshyna et al., 2021), as well as methods of excitotoxic neuronal damage.
Methods for recording changes in intracellular free calcium ion concentration and simultaneous measurement of evoked postsynaptic currents at individual CNS neuron synapses (Fedulova &Veselovsky, 2004). Methods for analyzing quantal neurotransmitter release at synapses of cultured neurons from the central and peripheral nervous systems of rats.
Method for investigating the electrophysiological properties of retinal ganglion cells in the intact retina, allowing maximal preservation of intracellular structures and cellular regulatory mechanisms under both normal conditions and experimental diabetes.
Microfluorimetric recording of changes in intracellular free calcium ion concentration in the cytosol of retinal ganglion cells in the intact retina during cellular activity or under the influence of various molecular substances (Kuznetsov et al., 2012).
Immunocytochemical analysis of neuronal protein expression after measuring somatic membrane currents to identify the neuron's neurotransmitter phenotype in cell culture (Grigorov et al., 2014).
Reverse transcription polymerase chain reaction (RT-PCR) method for detecting the expression of specific ion channel subunit genes in the whole hippocampus, in hippocampal neuron cultures, and in individual interneurons of hippocampal cultures and in retinal ganglion cells (Grigorov et al., 2014).
Method for recording action potentials from neurons of ganglia or autonomic nervous system plexuses using orthodromic and antidromic electrical stimulation of ganglion nerves or iontophoretic application of excitatory neurotransmitters directly onto the soma of ganglion neurons (Nastenko et al., 2022, 2024).
