DHSI:Methods

About Members Research Collaborators Methods History Selected Publications Grants Contacts Our methods Animal (rat) models of pathologies: Animal models Rotenone Parkinson desease model; Streptozotocin(STZ)-induced diabetes; Doxorubicin-indused oxidative stress; Anoxia-reoxigenation oxidative stress; Culture of neonatal cardiomyocytes (in collaboration). In our Department we do: Microscopy: Microscopy Transmitted light microscopy; Immunochemistry: Western blot; Western blot Biochemical: Determination of the oxidative and nitrosatitative stresses - hydrogen peroxide, superoxide radical, hydroxyl radical, diene conjugates, malonic dialdehyde in homogenates of the heart, aorta, medulla oblongata and suspension of mitochondria; Determination of the antioxidant system activity (SODs, catalase, GSH-Px) Polarographic investigation of tissue PO2 and mitochondrial respiration Polarographic investigation of tissue PO2 and mitochondrial respiration (V. Nosar, L. Bratus) The original set for studying of the pattern of breathing at small laboratory animals (V. Portnichenko, A. Sidorenko, M. Kolcheva) Studying of the pattern of breathing at small laboratory animals Methods of pathological conditions correction: Nonpharmacological - IHT (interval hypoxy training) Pharmacological – flavonoids, drugs, etc. Medico-biological investigations at the Ukrainian Antarctic Station Academician Vernadsky. Medico-biological investigations at the Ukrainian Antarctic Station Academician Vernadsky (E. Moiseyenko) Elbrus Medical and Biological Station of the International Center of astronomical and medical and environmental research of NAS of Ukraine at a height of 2100m in the Elbrus region. In collaboration with other Departments: Microscopy: Neonatal cardiomyocytes culture Fluorescence microscopy; Confocal laser-scanning microscopy; Molecular biology: RT-PCR Heterologous expression; Cells transformation; mRNA isolation; Polymerase chain reaction (PCR)