Інститут фізіології ім. О. О. Богомольця
Our methods
Animal (rat) models of pathologies:
- Rotenone Parkinson desease model;
- Streptozotocin(STZ)-induced diabetes;
- Doxorubicin-indused oxidative stress;
- Anoxia-reoxigenation oxidative stress;
- ulture of neonatal cardiomyocytes (in collaboration).
In our Department we do:
Microscopy:
- Transmitted light microscopy;
Immunochemistry:
Biochemical:
- Determination of the oxidative and nitrosatitative stresses - hydrogen peroxide, superoxide radical, hydroxyl radical, diene conjugates, malonic dialdehyde in homogenates of the heart, aorta, medulla oblongata and suspension of mitochondria;
- Determination of the antioxidant system activity (SODs, catalase, GSH-Px)
- Polarographic investigation of tissue PO2 and mitochondrial respiration
Studying of the pattern of breathing at small laboratory animals
Methods of pathological conditions correction:
- Nonpharmacological - IHT (interval hypoxy training)
- Pharmacological – flavonoids, drugs, etc.
Medico-biological investigations at the Ukrainian Antarctic Station Academician Vernadsky.
In collaboration with other Departments:
Microscopy:
- Fluorescence microscopy;
- Confocal laser-scanning microscopy;
Molecular biology:
- Heterologous expression;
- Cells transformation;
- mRNA isolation;
- Polymerase chain reaction (PCR)
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